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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [1]
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- Product number
- 36-4200 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Claudin 14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
Submitted references Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies.
Differential expression of claudin tight junction proteins in the human cortical nephron.
Expression and function of tight junctions in the crypt epithelium of human palatine tonsils.
Collins JE, Kirk A, Campbell SK, Mason J, Wilson SJ
Methods in molecular biology (Clifton, N.J.) 2011;762:371-82
Methods in molecular biology (Clifton, N.J.) 2011;762:371-82
Differential expression of claudin tight junction proteins in the human cortical nephron.
Kirk A, Campbell S, Bass P, Mason J, Collins J
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2010 Jul;25(7):2107-19
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2010 Jul;25(7):2107-19
Expression and function of tight junctions in the crypt epithelium of human palatine tonsils.
Go M, Kojima T, Takano K, Murata M, Ichimiya S, Tsubota H, Himi T, Sawada N
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2004 Dec;52(12):1627-38
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2004 Dec;52(12):1627-38
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry analysis of Claudin-14 showing staining in the membrane and cytoplasm of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin-14 polyclonal antibody (Product # 36-4200) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
