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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-92027 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARHGAP5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: EGLYRVSGNK TDQDNIQKQF DQDHNINLVS MEVTVNAVAG ALKAFFADLP DPLIPYSLHP ELLEAAKIPD KTERLHALKE IVKKFHPVNY DVFRYVITHL NRVSQQHKIN LMTADNLSIC FWPTLMRPDF ENREFLSTTK IHQSVVETFI QQCQFFFYNG EIVETTNIVA PPPPSNPGQL VEPMVPLQLP PPLQPQLIQP QLQTDPLGII; Positive Samples: HeLa, Jurkat, 293T, HepG2, PC-12, Mouse brain, Rat brain; Cellular Location: Cytoplasm, Membrane, Peripheral membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.15 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ARHGAP5 in extracts of various cell lines using ARHGAP5 Polyclonal Antibody (Product # PA5-92027) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ARHGAP5 was performed in HeLa cells using ARHGAP5 Polyclonal Antibody (Product # PA5-92027).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ARHGAP5 in paraffin-embedded rat brain using ARHGAP5 Polyclonal Antibody (Product # PA5-92027) at a dilution of 1:200.
