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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
- Flow cytometry [2]
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- Product number
- TA504240 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504240, RRID:AB_11126036
- Product name
- TLE2 mouse monoclonal antibody, clone OTI5A6 (formerly 5A6)
- Antibody type
- Monoclonal
- Description
- TLE2 mouse monoclonal antibody, clone OTI5A6 (formerly 5A6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TLE2
- Isotype
- IgG
- Antibody clone number
- OTI5A6
- Vial size
- 100 µl
- Concentration
- 0.85 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TLE2 (RC202474, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TLE2.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-TLE2 mouse monoclonal antibody (TA504240) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TLE2(RC202474).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TLE2 mouse monoclonal antibody. (TA504240)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TLE2 mouse monoclonal antibody. (TA504240)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TLE2 mouse monoclonal antibody. (TA504240)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TLE2 mouse monoclonal antibody. (TA504240)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TLE2 mouse monoclonal antibody. (TA504240)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TLE2 mouse monoclonal antibody. (TA504240)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TLE2 mouse monoclonal antibody. (TA504240)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-TLE2 antibody(TA504240),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC202474 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TLE2 antibody(TA504240), and then analyzed by flow cytometry.
- Validation comment
- FC
