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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF7216 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TLE2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human TLE2 in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) TLE1, rhTLE3, and rhTLE4 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q04725- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- TLE2 in Human Lung Mesothelioma. TLE2 was detected in immersion fixed paraffin-embedded sections of human lung mesothelioma using Sheep Anti-Human TLE2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7216) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
