H00001723-M01

antibody from Abnova Corporation

Targeting: DHODH

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001723-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001723-M01, RRID:AB_490083

Product name

DHODH monoclonal antibody (M01), clone 6E1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant DHODH.

Antigen sequence

GDERFYAEHLMPTLQGLLDPESAHRLAVRFTSLGL
LPRARFQDSDMLEVRVLGHKFRNPVGIAAGFDKHG
EAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVFRL
PEDQ


Isotype

IgG

Antibody clone number

6E1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Inhibition of pyrimidine biosynthesis pathway suppresses viral growth through innate immunity.

Lucas-Hourani M, Dauzonne D, Jorda P, Cousin G, Lupan A, Helynck O, Caignard G, Janvier G, André-Leroux G, Khiar S, Escriou N, Desprès P, Jacob Y, Munier-Lehmann H, Tangy F, Vidalain PO
PLoS pathogens 2013;9(10):e1003678

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

DHODH monoclonal antibody (M01), clone 6E1 Western Blot analysis of DHODH expression in MCF-7 ( Cat # L046V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged DHODH is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to DHODH on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol