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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- 14877-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DHODH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAWRHLKKR AQDAVIILGG GGLLFASYLM ATGDERFYAE HLMPTLQGLL DPESAHRLAV RFTSLGLLPR ARFQDSDMLE VRVLGHKFRN PVGIAAGFDK HGEAVDGLYK MGFGFVEIGS VTPKPQEGNP RPRVFRLPED QAVINRYGFN SHGLSVVEHR LRARQQKQAK LTEDGLPLGV NLGKNKTSVD AAEDYAEGVR VLGPLADYLV VNVSSPNTAG LRSLQGKAEL RRLLTKVLQE RDGLRRVHRP AVLVKIAPDL TSQDKEDIAS VVKELGIDGL IVTNTTVSRP AGLQGALRSE TGGLSGKPLR DLSTQTIREM YALTQGRVPI IGVGGVSSGQ DALEKIRAGA SLVQLYTALT FWGPPVVGKV KRELEALLKE QGFGGVTDAI GADHRR (1-395 aa encoded by B C065245)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14877-1-AP (DHODH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14877-1-AP (DHODH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 14877-1-AP (DHODH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat ovary tissue were subjected to SDS PAGE followed by western blot with 14877-1-AP (DHODH Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat spleen tissue were subjected to SDS PAGE followed by western blot with 14877-1-AP (DHODH Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using DHODH antibody 14877-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using DHODH antibody 14877-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 14877-1-AP (DHODH antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 14877-1-AP (DHODH antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DHODH antibody (Product # 14877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DHODH (IP:14877-1-AP, 4ug; Detection:14877-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.
