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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-117029 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FIS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: HeLa, 293T, MCF7
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.45 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from normal (control) and TTC11 / FIS1 Rabbit pAb knockout (KO) HeLa cells using FIS1 Polyclonal Antibody (Product # PA5-117029) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of 293T cells using FIS1 Polyclonal Antibody (Product # PA5-117029) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of FIS1 in HeLa cells using FIS1 Polyclonal Antibody (Product # PA5-117029) at a dilution of 1:50. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of FIS1 in rat heart tissue using FIS1 Polyclonal Antibody (Product # PA5-117029) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of FIS1 in rat heart tissue using FIS1 Polyclonal Antibody (Product # PA5-117029) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of FIS1 in mouse heart tissue using FIS1 Polyclonal Antibody (Product # PA5-117029) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of FIS1 in 200 µg extracts of MCF7 cells using FIS1 Polyclonal Antibody (Product # PA5-117029). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:100
