Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-117481 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RC74 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody has been tested in direct-ELISA
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 5 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RC74 using RC74 Polyclonal Antibody (Product # PA5-117481) at 3.4 µg/mL. Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 74, 72 kDa. Observed band size: 74, 72 kDa
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RC74 using RC74 Polyclonal Antibody (Product # PA5-117481) at 3.4 µg/mL. Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 74, 72 kDa. Observed band size: 74, 72 kDa
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RC74 in multiple sample types using a RC74 polyclonal antibody (Product # PA5-117481) at a dilution of 1:2,000. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: Hela whole cell lysate, Jurkat whole cell lysate, and 293 whole cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of RC74 in HeLa cells using RC74 Polyclonal Antibody (Product # PA5-117481) at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of RC74 in paraffin-embedded human prostate cancer using RC74 Polyclonal Antibody (Product # PA5-117481) diluted at 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of RC74 in paraffin-embedded human ovarian cancer using RC74 Polyclonal Antibody (Product # PA5-117481) diluted at 1:100.