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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- MA5-16144 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHD3 Monoclonal Antibody (EG188e/d5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- The target sequence has 98% sequence homology with bovine, rat and mouse.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- EG188e/d5
- Vial size
- 200 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Potential regulation of glioma through the induction of apoptosis signaling via Egl-9 family hypoxia-inducible factor 3.
Mao K, You C, Lei D, Zhang H
Oncology letters 2017 Feb;13(2):893-897
Oncology letters 2017 Feb;13(2):893-897
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHD3 using a monoclonal antibody (Product # MA5-16144).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHD3 in (1) Cobalt chloride treated COS-7 nuclear extracts and (2) Untreated COS-7 nuclear extracts. Samples were incubated in PHD3 monoclonal antibody (Product # MA5-16144).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHD3 in 0.5 mg/mL Hypoxic HeLa lysate. Samples were incubated in PHD3 monoclonal (Product # MA5-16144). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PHD3 in human renal cancer. Samples were incubated with PHD3 monoclonal antibody (Product # MA5-16144) followed by DAB with hematoxylin counterstain.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
