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LearnNB100-303
antibody from Novus Biologicals
Targeting: EGLN3
HIFPH3, PHD3
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [40]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- NB100-303 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-303, RRID:AB_10003302
- Product name
- Rabbit Polyclonal EGLN3/PHD3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Prolyl hydroxylase 3 controls the intestine goblet cell generation through stabilizing ATOH1.
Prolyl hydroxylase 3 stabilizes the p53 tumor suppressor by inhibiting the p53-MDM2 interaction in a hydroxylase-independent manner.
PHD3 regulates glucose metabolism by suppressing stress-induced signalling and optimising gluconeogenesis and insulin signalling in hepatocytes.
Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia.
PHD3 Acts as Tumor Suppressor in Mouse Osteosarcoma and Influences Tumor Vascularization via PDGF-C Signaling.
The Factor Inhibiting HIF Asparaginyl Hydroxylase Regulates Oxidative Metabolism and Accelerates Metabolic Adaptation to Hypoxia.
Estrogen receptor β2 induces proliferation and invasiveness of triple negative breast cancer cells: association with regulation of PHD3 and HIF-1α.
Dual role of MUC1 mucin in kidney ischemia-reperfusion injury: Nephroprotector in early phase, but pro-fibrotic in late phase.
Prolyl hydroxylase domain 2 deficiency promotes skeletal muscle fiber-type transition via a calcineurin/NFATc1-dependent pathway.
High-Density Lipoproteins Rescue Diabetes-Impaired Angiogenesis via Scavenger Receptor Class B Type I.
Oxygen-dependent Regulation of Erythropoietin Receptor Turnover and Signaling.
Cardiomyocyte-Specific Transgenic Expression of Prolyl-4-Hydroxylase Domain 3 Impairs the Myocardial Response to Ischemia.
Muc1 is protective during kidney ischemia-reperfusion injury.
Inhibition of HIF prolyl-4-hydroxylases by FG-4497 reduces brain tissue injury and edema formation during ischemic stroke.
Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions.
ERα upregulates Phd3 to ameliorate HIF-1 induced fibrosis and inflammation in adipose tissue.
Loss of PHD3 allows tumours to overcome hypoxic growth inhibition and sustain proliferation through EGFR.
PHD3 regulates EGFR internalization and signalling in tumours.
High-density lipoproteins augment hypoxia-induced angiogenesis via regulation of post-translational modulation of hypoxia-inducible factor 1α.
The role of prolyl hydroxylase domain protein (PHD) during rosiglitazone-induced adipocyte differentiation.
Negative regulation of HIF in skeletal muscle of elite endurance athletes: a tentative mechanism promoting oxidative metabolism.
Cellular overexpression of aquaporins slows down the natural HIF-2α degradation during prolonged hypoxia.
EGLN3 inhibition of NF-κB is mediated by prolyl hydroxylase-independent inhibition of IκB kinase γ ubiquitination.
Neuron-specific prolyl-4-hydroxylase domain 2 knockout reduces brain injury after transient cerebral ischemia.
Increased angiogenesis protects against adipose hypoxia and fibrosis in metabolic disease-resistant 11β-hydroxysteroid dehydrogenase type 1 (HSD1)-deficient mice.
Impaired hypoxic response in senescent mouse brain.
Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1.
Prolyl hydroxylase domain protein 3 targets Pax2 for destruction.
Prolyl hydroxylase domain (PHD) 2 affects cell migration and F-actin formation via RhoA/rho-associated kinase-dependent cofilin phosphorylation.
Molecular characterization of antibody epitopes of Ehrlichia chaffeensis ankyrin protein 200 and tandem repeat protein 47 and evaluation of synthetic immunodeterminants for serodiagnosis of human monocytotropic ehrlichiosis.
Toxoplasma gondii activates hypoxia-inducible factor (HIF) by stabilizing the HIF-1alpha subunit via type I activin-like receptor kinase receptor signaling.
A feedback loop involving the Phd3 prolyl hydroxylase tunes the mammalian hypoxic response in vivo.
HIF-prolyl hydroxylases in the rat kidney: physiologic expression patterns and regulation in acute kidney injury.
Proteasomal activity in placentas from women with preeclampsia and intrauterine growth restriction: implications for expression of HIF-alpha proteins.
Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure.
Regulation of adult erythropoiesis by prolyl hydroxylase domain proteins.
Hypoxia causes downregulation of mismatch repair system and genomic instability in stem cells.
The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress.
An alternatively spliced transcript of the PHD3 gene retains prolyl hydroxylase activity.
OS-9 interacts with hypoxia-inducible factor 1alpha and prolyl hydroxylases to promote oxygen-dependent degradation of HIF-1alpha.
Xu YM, Gao Q, Zhang JZ, Lu YT, Xing DM, Qin YQ, Fang J
Cell death and differentiation 2020 Jul;27(7):2131-2142
Cell death and differentiation 2020 Jul;27(7):2131-2142
Prolyl hydroxylase 3 stabilizes the p53 tumor suppressor by inhibiting the p53-MDM2 interaction in a hydroxylase-independent manner.
Xu Y, Gao Q, Xue Y, Li X, Xu L, Li C, Qin Y, Fang J
The Journal of biological chemistry 2019 Jun 21;294(25):9949-9958
The Journal of biological chemistry 2019 Jun 21;294(25):9949-9958
PHD3 regulates glucose metabolism by suppressing stress-induced signalling and optimising gluconeogenesis and insulin signalling in hepatocytes.
Yano H, Sakai M, Matsukawa T, Yagi T, Naganuma T, Mitsushima M, Iida S, Inaba Y, Inoue H, Unoki-Kubota H, Kaburagi Y, Asahara SI, Kido Y, Minami S, Kasuga M, Matsumoto M
Scientific reports 2018 Sep 24;8(1):14290
Scientific reports 2018 Sep 24;8(1):14290
Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia.
Chowdhury A, Aich A, Jain G, Wozny K, Lüchtenborg C, Hartmann M, Bernhard O, Balleiniger M, Alfar EA, Zieseniss A, Toischer K, Guan K, Rizzoli SO, Brügger B, Fischer A, Katschinski DM, Rehling P, Dudek J
Cell reports 2018 Oct 16;25(3):561-570.e6
Cell reports 2018 Oct 16;25(3):561-570.e6
PHD3 Acts as Tumor Suppressor in Mouse Osteosarcoma and Influences Tumor Vascularization via PDGF-C Signaling.
Egners A, Rezaei M, Kuzmanov A, Poitz DM, Streichert D, Müller-Reichert T, Wielockx B, Breier G
Cancers 2018 Dec 6;10(12)
Cancers 2018 Dec 6;10(12)
The Factor Inhibiting HIF Asparaginyl Hydroxylase Regulates Oxidative Metabolism and Accelerates Metabolic Adaptation to Hypoxia.
Sim J, Cowburn AS, Palazon A, Madhu B, Tyrakis PA, Macías D, Bargiela DM, Pietsch S, Gralla M, Evans CE, Kittipassorn T, Chey YCJ, Branco CM, Rundqvist H, Peet DJ, Johnson RS
Cell metabolism 2018 Apr 3;27(4):898-913.e7
Cell metabolism 2018 Apr 3;27(4):898-913.e7
Estrogen receptor β2 induces proliferation and invasiveness of triple negative breast cancer cells: association with regulation of PHD3 and HIF-1α.
Bialesova L, Xu L, Gustafsson JÅ, Haldosen LA, Zhao C, Dahlman-Wright K
Oncotarget 2017 Sep 29;8(44):76622-76633
Oncotarget 2017 Sep 29;8(44):76622-76633
Dual role of MUC1 mucin in kidney ischemia-reperfusion injury: Nephroprotector in early phase, but pro-fibrotic in late phase.
Gibier JB, Hémon B, Fanchon M, Gaudelot K, Pottier N, Ringot B, Van Seuningen I, Glowacki F, Cauffiez C, Blum D, Copin MC, Perrais M, Gnemmi V
Biochimica et biophysica acta. Molecular basis of disease 2017 Jun;1863(6):1336-1349
Biochimica et biophysica acta. Molecular basis of disease 2017 Jun;1863(6):1336-1349
Prolyl hydroxylase domain 2 deficiency promotes skeletal muscle fiber-type transition via a calcineurin/NFATc1-dependent pathway.
Shin J, Nunomiya A, Kitajima Y, Dan T, Miyata T, Nagatomi R
Skeletal muscle 2016;6:5
Skeletal muscle 2016;6:5
High-Density Lipoproteins Rescue Diabetes-Impaired Angiogenesis via Scavenger Receptor Class B Type I.
Tan JT, Prosser HC, Dunn LL, Vanags LZ, Ridiandries A, Tsatralis T, Lecce L, Clayton ZE, Yuen SC, Robertson S, Lam YT, Celermajer DS, Ng MK, Bursill CA
Diabetes 2016 Oct;65(10):3091-103
Diabetes 2016 Oct;65(10):3091-103
Oxygen-dependent Regulation of Erythropoietin Receptor Turnover and Signaling.
Heir P, Srikumar T, Bikopoulos G, Bunda S, Poon BP, Lee JE, Raught B, Ohh M
The Journal of biological chemistry 2016 Apr 1;291(14):7357-72
The Journal of biological chemistry 2016 Apr 1;291(14):7357-72
Cardiomyocyte-Specific Transgenic Expression of Prolyl-4-Hydroxylase Domain 3 Impairs the Myocardial Response to Ischemia.
Zieseniss A, Hesse AR, Jatho A, Krull S, Hölscher M, Vogel S, Katschinski DM
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2015;36(3):843-51
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2015;36(3):843-51
Muc1 is protective during kidney ischemia-reperfusion injury.
Pastor-Soler NM, Sutton TA, Mang HE, Kinlough CL, Gendler SJ, Madsen CS, Bastacky SI, Ho J, Al-Bataineh MM, Hallows KR, Singh S, Monga SP, Kobayashi H, Haase VH, Hughey RP
American journal of physiology. Renal physiology 2015 Jun 15;308(12):F1452-62
American journal of physiology. Renal physiology 2015 Jun 15;308(12):F1452-62
Inhibition of HIF prolyl-4-hydroxylases by FG-4497 reduces brain tissue injury and edema formation during ischemic stroke.
Reischl S, Li L, Walkinshaw G, Flippin LA, Marti HH, Kunze R
PloS one 2014;9(1):e84767
PloS one 2014;9(1):e84767
Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions.
Swain L, Wottawa M, Hillemann A, Beneke A, Odagiri H, Terada K, Endo M, Oike Y, Farhat K, Katschinski DM
Journal of leukocyte biology 2014 Sep;96(3):365-75
Journal of leukocyte biology 2014 Sep;96(3):365-75
ERα upregulates Phd3 to ameliorate HIF-1 induced fibrosis and inflammation in adipose tissue.
Kim M, Neinast MD, Frank AP, Sun K, Park J, Zehr JA, Vishvanath L, Morselli E, Amelotte M, Palmer BF, Gupta RK, Scherer PE, Clegg DJ
Molecular metabolism 2014 Sep;3(6):642-51
Molecular metabolism 2014 Sep;3(6):642-51
Loss of PHD3 allows tumours to overcome hypoxic growth inhibition and sustain proliferation through EGFR.
Henze AT, Garvalov BK, Seidel S, Cuesta AM, Ritter M, Filatova A, Foss F, Dopeso H, Essmann CL, Maxwell PH, Reifenberger G, Carmeliet P, Acker-Palmer A, Acker T
Nature communications 2014 Nov 25;5:5582
Nature communications 2014 Nov 25;5:5582
PHD3 regulates EGFR internalization and signalling in tumours.
Garvalov BK, Foss F, Henze AT, Bethani I, Gräf-Höchst S, Singh D, Filatova A, Dopeso H, Seidel S, Damm M, Acker-Palmer A, Acker T
Nature communications 2014 Nov 25;5:5577
Nature communications 2014 Nov 25;5:5577
High-density lipoproteins augment hypoxia-induced angiogenesis via regulation of post-translational modulation of hypoxia-inducible factor 1α.
Tan JT, Prosser HC, Vanags LZ, Monger SA, Ng MK, Bursill CA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Jan;28(1):206-17
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Jan;28(1):206-17
The role of prolyl hydroxylase domain protein (PHD) during rosiglitazone-induced adipocyte differentiation.
Kim J, Kwak HJ, Cha JY, Jeong YS, Rhee SD, Cheon HG
The Journal of biological chemistry 2014 Jan 31;289(5):2755-64
The Journal of biological chemistry 2014 Jan 31;289(5):2755-64
Negative regulation of HIF in skeletal muscle of elite endurance athletes: a tentative mechanism promoting oxidative metabolism.
Lindholm ME, Fischer H, Poellinger L, Johnson RS, Gustafsson T, Sundberg CJ, Rundqvist H
American journal of physiology. Regulatory, integrative and comparative physiology 2014 Aug 1;307(3):R248-55
American journal of physiology. Regulatory, integrative and comparative physiology 2014 Aug 1;307(3):R248-55
Cellular overexpression of aquaporins slows down the natural HIF-2α degradation during prolonged hypoxia.
Galán-Cobo A, Sánchez-Silva R, Serna A, Abreu-Rodríguez I, Muñoz-Cabello AM, Echevarría M
Gene 2013 Jun 10;522(1):18-26
Gene 2013 Jun 10;522(1):18-26
EGLN3 inhibition of NF-κB is mediated by prolyl hydroxylase-independent inhibition of IκB kinase γ ubiquitination.
Fu J, Taubman MB
Molecular and cellular biology 2013 Aug;33(15):3050-61
Molecular and cellular biology 2013 Aug;33(15):3050-61
Neuron-specific prolyl-4-hydroxylase domain 2 knockout reduces brain injury after transient cerebral ischemia.
Kunze R, Zhou W, Veltkamp R, Wielockx B, Breier G, Marti HH
Stroke 2012 Oct;43(10):2748-56
Stroke 2012 Oct;43(10):2748-56
Increased angiogenesis protects against adipose hypoxia and fibrosis in metabolic disease-resistant 11β-hydroxysteroid dehydrogenase type 1 (HSD1)-deficient mice.
Michailidou Z, Turban S, Miller E, Zou X, Schrader J, Ratcliffe PJ, Hadoke PW, Walker BR, Iredale JP, Morton NM, Seckl JR
The Journal of biological chemistry 2012 Feb 3;287(6):4188-97
The Journal of biological chemistry 2012 Feb 3;287(6):4188-97
Impaired hypoxic response in senescent mouse brain.
Rabie T, Kunze R, Marti HH
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 2011 Oct;29(6):655-61
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 2011 Oct;29(6):655-61
Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1.
Luo W, Hu H, Chang R, Zhong J, Knabel M, O'Meally R, Cole RN, Pandey A, Semenza GL
Cell 2011 May 27;145(5):732-44
Cell 2011 May 27;145(5):732-44
Prolyl hydroxylase domain protein 3 targets Pax2 for destruction.
Yan B, Jiao S, Zhang HS, Lv DD, Xue J, Fan L, Wu GH, Fang J
Biochemical and biophysical research communications 2011 Jun 3;409(2):315-20
Biochemical and biophysical research communications 2011 Jun 3;409(2):315-20
Prolyl hydroxylase domain (PHD) 2 affects cell migration and F-actin formation via RhoA/rho-associated kinase-dependent cofilin phosphorylation.
Vogel S, Wottawa M, Farhat K, Zieseniss A, Schnelle M, Le-Huu S, von Ahlen M, Malz C, Camenisch G, Katschinski DM
The Journal of biological chemistry 2010 Oct 29;285(44):33756-63
The Journal of biological chemistry 2010 Oct 29;285(44):33756-63
Molecular characterization of antibody epitopes of Ehrlichia chaffeensis ankyrin protein 200 and tandem repeat protein 47 and evaluation of synthetic immunodeterminants for serodiagnosis of human monocytotropic ehrlichiosis.
Luo T, Zhang X, Nicholson WL, Zhu B, McBride JW
Clinical and vaccine immunology : CVI 2010 Jan;17(1):87-97
Clinical and vaccine immunology : CVI 2010 Jan;17(1):87-97
Toxoplasma gondii activates hypoxia-inducible factor (HIF) by stabilizing the HIF-1alpha subunit via type I activin-like receptor kinase receptor signaling.
Wiley M, Sweeney KR, Chan DA, Brown KM, McMurtrey C, Howard EW, Giaccia AJ, Blader IJ
The Journal of biological chemistry 2010 Aug 27;285(35):26852-26860
The Journal of biological chemistry 2010 Aug 27;285(35):26852-26860
A feedback loop involving the Phd3 prolyl hydroxylase tunes the mammalian hypoxic response in vivo.
Minamishima YA, Moslehi J, Padera RF, Bronson RT, Liao R, Kaelin WG Jr
Molecular and cellular biology 2009 Nov;29(21):5729-41
Molecular and cellular biology 2009 Nov;29(21):5729-41
HIF-prolyl hydroxylases in the rat kidney: physiologic expression patterns and regulation in acute kidney injury.
Schödel J, Klanke B, Weidemann A, Buchholz B, Bernhardt W, Bertog M, Amann K, Korbmacher C, Wiesener M, Warnecke C, Kurtz A, Eckardt KU, Willam C
The American journal of pathology 2009 May;174(5):1663-74
The American journal of pathology 2009 May;174(5):1663-74
Proteasomal activity in placentas from women with preeclampsia and intrauterine growth restriction: implications for expression of HIF-alpha proteins.
Rajakumar A, Michael HM, Daftary A, Jeyabalan A, Gilmour C, Conrad KP
Placenta 2008 Mar;29(3):290-9
Placenta 2008 Mar;29(3):290-9
Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure.
Minamishima YA, Moslehi J, Bardeesy N, Cullen D, Bronson RT, Kaelin WG Jr
Blood 2008 Mar 15;111(6):3236-44
Blood 2008 Mar 15;111(6):3236-44
Regulation of adult erythropoiesis by prolyl hydroxylase domain proteins.
Takeda K, Aguila HL, Parikh NS, Li X, Lamothe K, Duan LJ, Takeda H, Lee FS, Fong GH
Blood 2008 Mar 15;111(6):3229-35
Blood 2008 Mar 15;111(6):3229-35
Hypoxia causes downregulation of mismatch repair system and genomic instability in stem cells.
Rodríguez-Jiménez FJ, Moreno-Manzano V, Lucas-Dominguez R, Sánchez-Puelles JM
Stem cells (Dayton, Ohio) 2008 Aug;26(8):2052-62
Stem cells (Dayton, Ohio) 2008 Aug;26(8):2052-62
The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress.
Mikhaylova O, Ignacak ML, Barankiewicz TJ, Harbaugh SV, Yi Y, Maxwell PH, Schneider M, Van Geyte K, Carmeliet P, Revelo MP, Wyder M, Greis KD, Meller J, Czyzyk-Krzeska MF
Molecular and cellular biology 2008 Apr;28(8):2701-17
Molecular and cellular biology 2008 Apr;28(8):2701-17
An alternatively spliced transcript of the PHD3 gene retains prolyl hydroxylase activity.
Cervera AM, Apostolova N, Luna-Crespo F, Sanjuan-Pla A, Garcia-Bou R, McCreath KJ
Cancer letters 2006 Feb 20;233(1):131-8
Cancer letters 2006 Feb 20;233(1):131-8
OS-9 interacts with hypoxia-inducible factor 1alpha and prolyl hydroxylases to promote oxygen-dependent degradation of HIF-1alpha.
Baek JH, Mahon PC, Oh J, Kelly B, Krishnamachary B, Pearson M, Chan DA, Giaccia AJ, Semenza GL
Molecular cell 2005 Feb 18;17(4):503-12
Molecular cell 2005 Feb 18;17(4):503-12
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: EGLN3/PHD3 Antibody [NB100-303] - Image shows a specific band for PHD3/HIF Prolyl Hydroxylase 3 in 0.5 mg/mL of Hypoxic HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EGLN3/PHD3 Antibody [NB100-303] - Total protein from Mouse MEF, Human RT4 and HeLa, and Rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD3 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EGLN3/PHD3 Antibody [NB100-303] - Whole cell protein from MEF cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/mL anti-EGLN3/PHD3 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EGLN3/PHD3 Antibody [NB100-303] - Tamoxifen administration-induced PHD2 deletion in skeletal muscle in Phd2f/f/Rosa26CreERT2 mice. Anti-PHD2 and PHD3 Western blotting of gastrocnemius and soleus muscles at 6 weeks after tamoxifen administration. Image collected and cropped by CiteAb from the following publication (http://www.skeletalmusclejournal.com/content/6/1/5), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody [NB100-303] - LDL Receptor was detected in immersion fixed paraffin-embedded sections of human liver cancer using rabbit anti-human antibody (NB100-303) at 1:3000 overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent (VC003) with DAB chromogen (brown) and counterstained with hematoxylin (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: EGLN3/PHD3 Antibody [NB100-303] - PHD3 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human EGLN3 polyclonal Antibody (NB100-303) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody (VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.
