10114-1-AP

antibody from Invitrogen Antibodies

Targeting: SNX6

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10114-1-AP

Provider

Invitrogen Antibodies

Product name

SNX6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: AIFKKTVAM HEVFLCRVAA HPILRRDLNF HVFLEYNQDL SVRGKNKKEK LEDFFKNMVK SADGVIVSGV KDVDDFFEHE RTFLLEYHNR VKDASAKSDR MTRSHKSAAD DYNRIGSSLY ALGTQDSTDI CKFFLKVSEL FDKTRKIEAR VSADEDLKLS DLLKYYLRES QAAKDLLYRR SRSLVDYENA NKALDKARAK NKDVLQAETS QQLCCQKFEK ISESAKQELI DFKTRRVAAF RKNLVELAEL ELKHAKGNLQ LLQNCLAVLN GDT (19-290 aa encoded by BC001798)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human placenta tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP ( SNX6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart slide using 10114-1-AP ( SNX6 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart slide using 10114-1-AP ( SNX6 Antibody) at dilution of 1:50 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 RAW 264.7 cells were stained with 0.2ug SNX6 antibody (10114-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SNX6 (IP:10114-1-AP, 4ug; Detection:10114-1-AP 1:600) with HepG2 cells lysate 4000ug.