17460-1-AP

antibody from Invitrogen Antibodies

Targeting: IFT140 gs114, KIAA0590, WDTC2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17460-1-AP

Provider

Invitrogen Antibodies

Product name

IFT140 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: AEDLDETSD PALLARCSDF FIEHSQYERA VELLLAARKY QEALQLCLGQ NMSITEEMAE KMTVAKDSSD LPEESRRELL EQIADCCMRQ GSYHLATKKY TQAGNKLKAM RALLKSGDTE KITFFASVSR QKEIYIMAAN YLQSLDWRKE PEIMKNIIGF YTKGRALDLL AGFYDACAQV EIDEYQNYDK AHGALTEAYK CLAKAKAKSP LDQETRLAQL QSRMALVKRF IQARRTYTED PKESIKQCEL LLEEPDLDST IRIGDVYGFL VEHYVRKEEY QTAYRFLEEM RRRLPLANMS YYVSPQAVDA VHRGLGLPLP RTVPEQVRHN SMEDARELDE EVVEEADDDP (1114-1462 aa encoded by BC035577)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.49 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP ( IFT140 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF result (enrichment to the base (arrowhead) and tip (asterix) of cilia) of anti-IFT140 (17460-1-AP, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 17460-1-AP ( IFT140 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 17460-1-AP ( IFT140 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using IFT140 antibody (Product # 17460-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). DAPI (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-IFT140 (IP: 17460-1-AP, 4 µg; Detection: 17460-1-AP 1:300) with rat testis tissue lysate 4400 µg.