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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-31176 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GNA15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: THP-1.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GNA15 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GNA15 Polyclonal Antibody (Product # PA5-31176) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GNA15 Polyclonal Antibody detects GNA15 protein by Western blot analysis. A. 30 µg THP-1 whole cell lysate/extract.10 % SDS-PAGE. GNA15 Polyclonal Antibody (Product # PA5-31176) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of G protein alpha 16 in paraformaldehyde-fixed HeLa cells using a G protein alpha 16 polyclonal antibody (Product # PA5-31176) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GNA15 Polyclonal Antibody detects G protein alpha 16 protein at cytoplasm on CX-1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded CX-1 xenograft. GNA15 Polyclonal Antibody (Product # PA5-31176) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
