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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00004782-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004782-M01, RRID:AB_425564
- Product name
- NFIC monoclonal antibody (M01), clone 1C12-2A2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant NFIC.
- Antigen sequence
MYSSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQ
ARKRKYFKKHEKRMSKDEERAVKDELLGEKPEVKQ
KWASRLLAKLRKDIRPECREDFVLSITGKKAPGCV
LSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKG
IPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKE
LDLYLAYFVRERDAEQSGSPRTGMGSDQEDSKPIT
LDTTDFQESFVTSGVFSVTELIQVSRTPVVTGTGP
NFSLGELQGHLAYDLNPASTGLRRTLPSTSSSGSK
RHKSGSMEEDVDTSPGGDYYTSPSSPTSSCRNWTE
DMEGGISSPVKKTEMDKSPFNSPSPQDSPRLSSFT
QHHRPVIAVHSGIARSPHPSSALHFPTTSILPQTA
STYFPHTAIRYPPHLNPQDPLKDLVSLACDPASQQ
PGPSWYLG- Isotype
- IgG
- Antibody clone number
- 1C12-2A2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references NFI-C2 temporal-spatial expression and cellular localization pattern during tooth formation.
Lamani E, Gluhak-Heinrich J, MacDougall M
Development, growth & differentiation 2015 Dec;57(9):625-38
Development, growth & differentiation 2015 Dec;57(9):625-38
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NFIC monoclonal antibody (M01), clone 1C12-2A2 Western Blot analysis of NFIC expression in A-431 ( Cat # L015V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.Lane 1: NFIC transfected lysate(47.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to NFIC on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
