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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-109295 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFIC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: A549, SiHa, A431.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nfic in Positive control: Lane 1: A549, Lane 2: SiHa. Samples were incubated with Nfic polyclonal antibody (Product # PA5-109295), at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nfic in Positive control: Lane 1: A549, Lane 2: SiHa. Samples were incubated with Nfic polyclonal antibody (Product # PA5-109295), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Nfic in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nfic polyclonal antibody (Product # PA5-109295), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Nfic in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nfic polyclonal antibody (Product # PA5-109295), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Nfic in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nfic polyclonal antibody (Product # PA5-109295), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nfic in paraffin-embedded human liver tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH6, 20 min), incubated with Nfic polyclonal antibody (Product # PA5-109295), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Nfic in SiHa cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Nfic polyclonal antibody (Product # PA5-109295) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
