RQ5664

antibody from NSJ Bioreagents

Targeting: NFIC CTF, CTF5, NF-I, NFI

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

RQ5664

Provider

NSJ Bioreagents

Product name

NFIC Antibody /

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Storage

After reconstitution, the NFIC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) U-2 OS, 3) T-47D, 4) PC-3, 5) HepG2, 6) A431, 7) Caco-2, 8) rat skeletal muscle and 9) mouse RAW264.7 lysate with NFIC antibody. Expected molecular weight: 49-74 kDa (isoforms).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human intestinal cancer with NFIC antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE mouse skeletal muscle with NFIC antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human pancreatic cancer with NFIC antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse skeletal muscle with NFIC antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat pancreas with NFIC antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.