16399-1-AP

antibody from Invitrogen Antibodies

Targeting: NFIC CTF, CTF5, NF-I, NFI

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16399-1-AP

Provider

Invitrogen Antibodies

Product name

NFIC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MYSSPLCLT QDEFHPFIEA LLPHVRAFAY TWFNLQARKR KYFKKHEKRM SKDEERAVKD ELLGEKPEVK QKWASRLLAK LRKDIRPECR EDFVLSITGK KAPGCVLSNP DQKGKMRRID CLRQADKVWR LDLVMVILFK GIPLESTDGE RLVKAAQCGH PVLCVQPHHI GVAVKELDLY LAYFVRERDA EQSGSPRTGM GSDQEDSKPI TLDTTDFQES FVTSGVFSVT ELIQVSRTPV VTGTGPNFSL GELQGHLAYD LNPASTGLRR TLPSTSSSGS KRHKSGSMEE DVDTSPGGDY YTSPSSPTSS SRNWTEDMEG GISSPVKKTE MDKSPFNSPS PQDSPRLSSF TQ (1-351 aa encoded by BC012120)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 16399-1-AP ( NFIC Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed PC-3 cells using 16399-1-AP (NFIC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NFIC (IP:16399-1-AP, 4ug; Detection:16399-1-AP 1:1000) with HeLa cells lysate 3600ug.