Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-31588 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMEM66 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, Jurkat, Raji.
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Transmembrane protein 66 using 30 µg of Jurkat lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Transmembrane protein 66 polyclonal antibody (Product # PA5-31588) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TMEM66 Polyclonal Antibody (Product # PA5-31588). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TMEM66 Polyclonal Antibody (Product # PA5-31588) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Transmembrane protein 66 in methanol-fixed HeLa cells using a Transmembrane protein 66 polyclonal antibody (Product # PA5-31588) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using Transmembrane protein 66 (Product # PA5-31588) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
