NBP2-20112

antibody from Novus Biologicals

Targeting: RBPMS HERMES

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-20112

Provider

Novus Biologicals

Product name

Rabbit Polyclonal RBPMS Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Nicotinamide and WLD(S) Act Together to Prevent Neurodegeneration in Glaucoma.

Williams PA, Harder JM, Foxworth NE, Cardozo BH, Cochran KE, John SWM
Frontiers in neuroscience 2017;11:232

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GlyCAM1 negatively regulates monocyte entry into the optic nerve head and contributes to radiation-based protection in glaucoma.

Williams PA, Braine CE, Foxworth NE, Cochran KE, John SWM
Journal of neuroinflammation 2017 Apr 26;14(1):93

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RBPMS Antibody [NBP2-20112] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RBPMS Antibody [NBP2-20112] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RBPMS Antibody [NBP2-20112] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RBPMS Antibody [NBP2-20112] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RBPMS Antibody [NBP2-20112] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] - Paraffin-embedded rat colon. RBPMS antibody diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] - Paraffin-embedded rat duodenum. RBPMS antibody diluted at 1:500

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] - Paraffin-embedded mouse retina. Green: RBPMS protein stained by RBPMS antibody diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] - Rat colon. RBPMS stained by RBPMS antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] - Mouse intestine. RBPMS stained by RBPMS antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] - Rat lymph node. RBPMS stained by RBPMS antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.