- PA5-31231 - Invitrogen Antibodies RBPMS antibody

Submitted references Genetic inhibition of collapsin response mediator protein-2 phosphorylation ameliorates retinal ganglion cell death in normal-tension glaucoma models.

Brahma MM, Takahashi K, Namekata K, Harada T, Goshima Y, Ohshima T
Genes to cells : devoted to molecular & cellular mechanisms 2022 Aug;27(8):526-536

Genetic inhibition of CRMP2 phosphorylation at serine 522 promotes axonal regeneration after optic nerve injury.

Kondo S, Takahashi K, Kinoshita Y, Nagai J, Wakatsuki S, Araki T, Goshima Y, Ohshima T
Scientific reports 2019 May 10;9(1):7188

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RBPMS using 30 µg of A549 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RBPMS polyclonal antibody (Product # PA5-31231) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RBPMS using 50 µg of mouse heart lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RBPMS polyclonal antibody (Product # PA5-31231) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RBPMS was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a RBPMS Polyclonal Antibody (Product # PA5-31231) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of RBPMS was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a RBPMS Polyclonal Antibody (Product # PA5-31231) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using RBPMS Polyclonal Antibody (Product # PA5-31231). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS Polyclonal Antibody (Product # PA5-31231) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of RBPMS was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a RBPMS Polyclonal Antibody (Product # PA5-31231) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using RBPMS Polyclonal Antibody (Product # PA5-31231). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS Polyclonal Antibody (Product # PA5-31231) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of RBPMS was performed in A549 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RBPMS Polyclonal Antibody (Product # PA5-31231) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of RBPMS was performed in paraffin-embedded rat duodenum tissue using RBPMS Polyclonal Antibody (Product # PA5-31231) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of RBPMS was performed in paraffin-embedded rat colon tissue using RBPMS Polyclonal Antibody (Product # PA5-31231) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of RBPMS was performed in paraffin-embedded mouse intestine tissue using RBPMS Polyclonal Antibody (Product # PA5-31231) at a dilution of 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of RBPMS was performed in paraffin-embedded rat lymph node tissue using RBPMS Polyclonal Antibody (Product # PA5-31231) at a dilution of 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of RBPMS was performed in paraffin-embedded rat colon tissue using RBPMS Polyclonal Antibody (Product # PA5-31231) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of RBPMS was performed in paraffin-embedded mouse retina tissue. Green: RBPMS stained by RBPMS Polyclonal Antibody (Product # PA5-31231) at a dilution of 1:250. Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 1 FIGURE Production of p25 after N-methyl-D-aspartate (NMDA) injection. (a, b) Dose-dependent decrease in the number of retinal ganglion cells (RGCs) observed 1 week after the injection of phosphate-buffered saline (PBS) or NMDA in 5 or 20 nmol concentrations. The retina samples are stained with anti-RNA-binding protein with multiple splicing, a specific marker for RGCs (Rodriguez et al., 2015). (c) Time course of p25 production within 24 h and amounts of p35 and p25 in retina samples from PBS or 20 nmol NMDA injected in the eye indicated time points by western blot analysis. (d) Quantification of p25/p35+p25 level ( n = 4, *** p < .001; * p < .05)

PA5-31231

Antibody data