Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-113509 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FBXO4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.47 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FBXO4 in multiple sample types using a FBXO4 polyclonal antibody (Product # PA5-113509) at a concentration of 3.1 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:10,000. Sample types that were tested include: Lane 1: 293T whole cell lysate, Lane 2: HepG2 whole cell lysate, Lane 3: HL60 whole cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded FBXO4 in human brain tissue using a FBXO4 Polyclonal antibody (Product # PA5-113509) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded FBXO4 in human heart tissue using a FBXO4 Polyclonal antibody (Product # PA5-113509) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of FBXO4 in HepG2 whole cell lysate using a Polyclonal antibody (Product #PA5-113509). An HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1:50,000). Lane 1: Rabbit control IgG, Lane 2: 4 µg primary antibody and 500 µg of HepG2 whole cell lysate, Lane 3: 20 µg of HepG2 whole cell lysate.