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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF1360 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Growth Hormone R/GHR Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Growth Hormone R/GHR in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant rat GHR is observed and approximately 20% cross-reactivity with recombinant human GHR is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q3UP14- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Molecular interactions of EphA4, growth hormone receptor, Janus kinase 2, and signal transducer and activator of transcription 5B.
Growth hormone is increased in the lungs and enhances experimental lung metastasis of melanoma in DJ-1 KO mice.
Hepatic growth hormone resistance after acute injury.
Loss of STAT5 causes liver fibrosis and cancer development through increased TGF-{beta} and STAT3 activation.
Targeted deletion of hepatic Igf1 in TRAMP mice leads to dramatic alterations in the circulating insulin-like growth factor axis but does not reduce tumor progression.
Sawada T, Arai D, Jing X, Miyajima M, Frank SJ, Sakaguchi K
PloS one 2017;12(7):e0180785
PloS one 2017;12(7):e0180785
Growth hormone is increased in the lungs and enhances experimental lung metastasis of melanoma in DJ-1 KO mice.
Chien CH, Lee MJ, Liou HC, Liou HH, Fu WM
BMC cancer 2016 Nov 8;16(1):871
BMC cancer 2016 Nov 8;16(1):871
Hepatic growth hormone resistance after acute injury.
Corrick RM, Li L, Frank SJ, Messina JL
Endocrinology 2013 Apr;154(4):1577-88
Endocrinology 2013 Apr;154(4):1577-88
Loss of STAT5 causes liver fibrosis and cancer development through increased TGF-{beta} and STAT3 activation.
Hosui A, Kimura A, Yamaji D, Zhu BM, Na R, Hennighausen L
The Journal of experimental medicine 2009 Apr 13;206(4):819-31
The Journal of experimental medicine 2009 Apr 13;206(4):819-31
Targeted deletion of hepatic Igf1 in TRAMP mice leads to dramatic alterations in the circulating insulin-like growth factor axis but does not reduce tumor progression.
Anzo M, Cobb LJ, Hwang DL, Mehta H, Said JW, Yakar S, LeRoith D, Cohen P
Cancer research 2008 May 1;68(9):3342-9
Cancer research 2008 May 1;68(9):3342-9
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Growth Hormone R/GHR in Mouse Liver. Growth Hormone R/GHR was detected in perfusion fixed frozen sections of mouse liver using Mouse Growth Hormone R/GHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1360) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
