- UM500090 - OriGene MRPS34 antibody

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Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunofluorescent staining of HeLa cells using anti-MRPS34 mouse monoclonal antibody (UM500090, green, 1:100). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue).
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRPS34 mouse monoclonal antibody. (UM500090; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MRPS34 mouse monoclonal antibody. (UM500090; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MRPS34mouse monoclonal antibody. (UM500090; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MRPS34 mouse monoclonal antibody. (UM500090; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human breast normal adjacent using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM500090 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in breast epithelia cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human brain using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM500090 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in .
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human pituitary using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM500090 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in breast epithelia cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human colon cancer using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM500090 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in the tumor cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human colon normal adjacent using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM500090 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in the normal epithelia cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human kidney normal adjacent using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM500090 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in the normal kidney epithelia cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human liver normal adjacent using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM500090 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and puntate cytoplasmic staining in hepatocyte cells
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human lung cancer using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM500090 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in tumor cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human thyroid cancer using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM500090 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in tumor cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-MRPS34 mouse monoclonal antibody (UM500090). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
Validation comment: 10K-CHIP

UM500090