NBP2-16861

antibody from Novus Biologicals

Targeting: HMGCS1 HMGCS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-16861

Provider

Novus Biologicals

Product name

Rabbit Polyclonal HMGCS1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HMGCS1 Antibody [NBP2-16861] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE gel, diluted at 1:5000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HMGCS1 Antibody [NBP2-16861] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 7. 5% SDS PAGE gel, diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HMGCS1 Antibody [NBP2-16861] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HMGCS1 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HMGCS1 Antibody [NBP2-16861] - Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HMGCS1 Antibody [NBP2-16861] - Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using antibody at 1:500 dilution.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HMGCS1 Antibody [NBP2-16861] - Mouse intestine. HMGCS1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HMGCS1 Antibody [NBP2-16861] - Mouse liver. HMGCS1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.