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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 17643-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMGCS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: TGGVGAVAL LIGPNAPLIF ERGLRGTHMQ HAYDFYKPDM LSEYPIVDGK LSIQCYLSAL DRCYSVYCKK IHAQWQKEGN DKDFTLNDFG FMIFHSPYCK LVQKSLARML LNDFLNDQNR DKNSIYSGLE AFGDVKLEDT YFDRDVEKAF MKASSELFSQ KTKASLLVSN QNGNMYTSSV YGSLASVLAQ YSPQQLAGKR IGVFSYGSGL AATLYSLKVT QDATPGSALD KITASLCDLK SRLDSRTGVA PDVFAENMKL REDTYHLVNY IPQGSIDSLF EGTWYLVRVD EKHRRTYARR PTPNDDTLDE GVGLVHSNIA TEHIPSPAKK VPRLPATAAE PEAAVISNGE H (171-520 aa encoded by BC083514)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 17643-1-AP (HMGCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 17643-1-AP (HMGCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of HMGCS1 antibody (17643-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMGCS1 transfected HepG2 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 17643-1-AP (HMGCS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using HMGCS1 antibody 17643-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-HMGCS1 (IP:17643-1-AP, 3ug; Detection:17643-1-AP 1:1000) with mouse liver tissue lysate 4000ug.
