PAB29445

antibody from Abnova Corporation

Targeting: OIP5 CT86, hMIS18beta, MIS18B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29445

Provider

Abnova Corporation

Product name

OIP5 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human OIP5.

Antigen sequence

CGSCGIPVGFHLYSTHAALAALRGHFCLSSDKMVC
YLLKTKAIVNASEMDIQNVPLSEKIAELKEKIVLT
HNRLKSLMKILSEVT


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG sp with OIP5 polyclonal antibody (Cat # PAB29445) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line PC-3 with OIP5 polyclonal antibody (Cat # PAB29445) at 1-4 ug/mL concentration shows positivity in cytoplasm and nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with OIP5 polyclonal antibody (Cat # PAB29445) shows strong cytoplasmic positivity in subset of cells in seminiferous ducts and in Leydig cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)