14524-1-AP

antibody from Invitrogen Antibodies

Targeting: MYBBP1A FLJ37886, P160, PAP2, Pol5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14524-1-AP

Provider

Invitrogen Antibodies

Product name

MYBBP1A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: STALSSFLT KRNSPLTVPM FLSLFSRHPV LCQSLLPILV QHITGPVRPR RQACLLLQKT LSMREVRSCF EDPEWKQLMG QVLAKVTENL RVLGEAQTKA QHQQALSSLE LLNVLFRTCK HEKLTLDLTV LLGVLQGQQQ SLQQGAHSTG SSRLHDLYWQ AMKTLGVQRP KLEKKDAKEI PSATQSPISK KRKKKGFLPE TKKRKKRKSE DGTPAEDGTP AATGGSQPPS MGRKKRNRTK AKVPAQANGT PTTKSPAPGA PTRSPSTPAK SPKLQKKNQK PSQVNGAPGS PTEPAGQKQH QKALPKKGVL GKSPLSALAR KKARLSLVIR SPSLLQSGAK KKAQVRKAGK P (979-1328 aa encoded by BC050546)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.35 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 14524-1-AP (MYBBP1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 14524-1-AP (MYBBP1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using MYBBP1A antibody 14524-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 14524-1-AP (MYBBP1A antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 14524-1-AP (MYBBP1A antibody) at dilution of 1:100 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug MYBBP1A antibody (14524-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MYBBP1A (IP:14524-1-AP, 4ug; Detection:14524-1-AP 1:500) with HEK-293 cells lysate 2800ug.