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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-18221 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ORP5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine, mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references 2-Hydroxypropyl-gamma-cyclodextrin overcomes NPC1 deficiency by enhancing lysosome-ER association and autophagy.
Singhal A, Krystofiak ES, Jerome WG, Song B
Scientific reports 2020 May 26;10(1):8663
Scientific reports 2020 May 26;10(1):8663
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human spleen lysate using Product # PA5-18221 at a concentration of 2 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ORP5 by a ORP5 monoclonal antibody (Product # PA5-18221) at a concentration of 0.2 µg/mL. Human Spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 HPgammaCD enhances lysosome-ER association in NPC1 fibroblasts. NPC1 mutant cells were treated with HPgammaCD or HPbetaCD (1 mM, 72 h). Cells were then analyzed for the levels of lysosome and ER contact proteins by immunoblotting ( a ) and for lysosome-ER association by confocal microscopy ( b-e ). The blots are from different parts of the same gel and delineated with dividing lines. Confocal microscopy was used to visualize the lysosome and ER by immunostaining cells for the lysosomal membrane protein (LAMP1, green) and the ER protein (Calreticulin or ORP5, red). Microscopic images showed co-localization of LAMP1 and CALR ( b ) or ORP5 ( d ) as a yellow color (merge). Nuclei were stained using DAPI (blue). The co-localization, measured by Pearson's correlation coefficient, was significantly higher in the CD-treated cells compared to untreated control cells ( c,e ). Data are mean +- S.E.M. of triplicates and a representative of three independent experiments. Symbols indicate the relative level of significance compared with the control (*P < 0.05, ***P < 0.001). Scale bar = 50 um.
