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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX102807 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102807, RRID:AB_11179180
- Product name
- Islet 1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HL-60 10% SDS PAGE GTX102807 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Islet 1 antibody detects Islet 1 protein by western blot analysis. Jurkat whole cell extracts and extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody (GTX102807) diluted at 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Islet 1 antibody detects Islet 1 protein by western blot analysis. K562 whole cell extracts and extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody (GTX102807) diluted at 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SH-SY5Y whole cell and nuclear extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody (GTX128201) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody (GTX102807) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Islet 1 antibody detects Islet 1 protein at nucleus by immunofluorescent analysis.Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Islet 1 protein stained by Islet 1 antibody (GTX102807) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Islet 1 antibody detects Islet 1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Islet 1 antibody (GTX102807) dilution: 1:100.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Islet 1 antibody detects Islet 1 protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: Islet 1 protein stained by Islet 1 antibody (GTX102807) diluted at 1:250.Red: Protein kinase C alpha staining.Blue: Fluoroshield with DAPI (GTX30920).
