15661-1-AP

antibody from Invitrogen Antibodies

Targeting: ISL1 Isl-1, ISLET1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15661-1-AP

Provider

Invitrogen Antibodies

Product name

Islet 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MGDMGDPPK KKRLISLCVG CGNQIHDQYI LRVSPDLEWH AACLKCAECN QYLDESCTCF VRDGKTYCKR DYIRLYGIKC AKCSIGFSKN DFVMRARSKV YHIECFRCVA CSRQLIPGDE FALREDGLFC RADHDVVERA SLGAGDPLSP LHPARPLQMA AEPISARQPA LRPHVHKQPE KTTRVRTVLN EKQLHTLRTC YAANPRPDAL MKEQLVEMTG LSPRVIRVWF QNKRCKDKKR SIMMKQLQQQ QPNDKTNIQG MTGTPMVAAS PERHDGGLQA NPVEVQSYQP PWKVLSDFAL QSDIDQPAFQ QLVNFSEGGP GSNSTGSEVA SMSSQLPDTP NSMVASPIEA (1-349 aa encoded by BC031213)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.29 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15661-1-AP (ISL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15661-1-AP (ISL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 15661-1-AP ( Islet 1 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 15661-1-AP ( Islet 1 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 15661-1-AP (Islet 1 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 15661-1-AP (Islet 1 Antibody) at dilution of 1:200 (under 10x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 µg Anti-Human Islet 1 (Product # 15661-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.2 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Islet 1 (IP:15661-1-AP, 4ug; Detection:15661-1-AP 1:500) with HeLa cells lysate 2000ug.