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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- GTX100308 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100308, RRID:AB_1240823
- Product name
- FOXA1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Resources for the Comprehensive Discovery of Functional RNA Elements.
Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13
Molecular cell 2016 Mar 17;61(6):903-13
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HepG2 (GTX27900) 10% SDS PAGE GTX100308 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXA1(GTX100308) antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FOXA1 antibody immunoprecipitates FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 £gg whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : Control with 2.5 £gg of pre-immune rabbit IgG C : Immunoprecipitation of FOXA1 by 2.5 £gg of FOXA1 antibody (GTX100308) 10% SDS-PAGE The immunoprecipitated FOXA1 protein was detected by FOXA1 antibody (GTX100308) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FOXA1 antibody detects FOXA1 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. FOXA1 antibody (GTX100308) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FOXA1 antibody detects FOXA1 protein at nucleus on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. FOXA1 antibody (GTX100308) dilution: 1:500.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 £gg of either control rabbit IgG or anti-FOXA1 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.
