Explore
Validate
LearnNBP1-30877
antibody from Novus Biologicals
Targeting: FOXA1
HNF3A
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-30877 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-30877, RRID:AB_2104839
- Product name
- Rabbit Polyclonal HNF-3 alpha/FoxA1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 ug whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of FOXA1 by 2.5 ug of FOXA1 antibody 10% SDS-PAGE The immunoprecipitated FOXA1 protein was detected by FOXA1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Paraffin-embedded human cervical carcinoma. FOXA1 antibody dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Paraffin-embedded human breast carcinoma. FOXA1 antibody dilution: 1:500.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-FOXA1 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.
