Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AM31963PU-N - Provider product page
- Provider
- OriGene
- Product name
- Cytochrome b5 (CYB5A) (1-135) mouse monoclonal antibody, clone 1A8, Purified
- Antibody type
- Monoclonal
- Description
- Cytochrome b5 (CYB5A) (1-135) mouse monoclonal antibody, clone 1A8, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CYB5A
- Isotype
- IgG
- Antibody clone number
- 1A8
- Vial size
- 50 µg
- Concentration
- 0.5 mg/ml
Submitted references Development of monoclonal antibodies against the human 3β-hydroxysteroid dehydrogenase/isomerase isozymes.
Gomez-Sanchez CE, Lewis M, Nanba K, Rainey WE, Kuppusamy M, Gomez-Sanchez EP
Steroids 2017 Nov;127:56-61
Steroids 2017 Nov;127:56-61
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of CYB5A expression in transfected 293T cell line by CYB5A monoclonal antibody (M06), clone 1A8.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CYB5A is 0.1 ng/ml as a capture antibody.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation of CYB5A transfected lysate using anti-CYB5A monoclonal antibody and Protein A Magnetic Bead.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Validation comment
- IHC