Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX120446 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX120446, RRID:AB_10731367
- Product name
- PELO antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation.
Knight JR, Bastide A, Roobol A, Roobol J, Jackson TJ, Utami W, Barrett DA, Smales CM, Willis AE
The Biochemical journal 2015 Jan 15;465(2):227-38
The Biochemical journal 2015 Jan 15;465(2):227-38
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: Jurkat 10% SDS PAGE GTX120446 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PELO antibody detects PELO protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with PELO antibody (GTX120446) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with PELO antibody (GTX120446) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with PELO antibody (GTX120446) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PELO antibody detects PELO protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PELO protein stained by PELO antibody (GTX120446) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using PELO(GTX120446) antibody at 1:500 dilution.
