FAB138P-025

antibody from R&D Systems

Targeting: KLRC2 CD159c, NKG2-C

Provider product page for FAB138P-025

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [37]
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Validations
Flow cytometry [1]

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Product number: FAB138P-025 - Provider product page
Provider: R&D Systems
Product name: Human NKG2C/CD159c PE-conjugated Antibody
Antibody type: Monoclonal
Description: Protein A or G purified from hybridoma culture supernatant. Detects human NKG2C/CD159c heterodimer with CD94 in flow cytometry. It does not cross-react with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 134591
Vial size: 25 Tests
Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

KLRC2 protein structure - FAB138P-025 shown in red.

Submitted references Between Innate and Adaptive Immune Responses: NKG2A, NKG2C, and CD8⁺ T Cell Recognition of HLA-E Restricted Self-Peptides Acquired in the Absence of HLA-Ia.

Pump WC, Kraemer T, Huyton T, Hò GT, Blasczyk R, Bade-Doeding C
International journal of molecular sciences 2019 Mar 22;20(6)

Suppression of costimulation by human cytomegalovirus promotes evasion of cellular immune defenses.

Wang ECY, Pjechova M, Nightingale K, Vlahava VM, Patel M, Ruckova E, Forbes SK, Nobre L, Antrobus R, Roberts D, Fielding CA, Seirafian S, Davies J, Murrell I, Lau B, Wilkie GS, Suárez NM, Stanton RJ, Vojtesek B, Davison A, Lehner PJ, Weekes MP, Wilkinson GWG, Tomasec P
Proceedings of the National Academy of Sciences of the United States of America 2018 May 8;115(19):4998-5003

Complete Remission with Reduction of High-Risk Clones following Haploidentical NK-Cell Therapy against MDS and AML.

Björklund AT, Carlsten M, Sohlberg E, Liu LL, Clancy T, Karimi M, Cooley S, Miller JS, Klimkowska M, Schaffer M, Watz E, Wikström K, Blomberg P, Wahlin BE, Palma M, Hansson L, Ljungman P, Hellström-Lindberg E, Ljunggren HG, Malmberg KJ
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Apr 15;24(8):1834-1844

The Clinical Features of Patients with Chronic Hepatitis C Virus Infections Are Associated with Killer Cell Immunoglobulin-Like Receptor Genes and Their Expression on the Surface of Natural Killer Cells.

Podhorzer A, Dirchwolf M, Machicote A, Belen S, Montal S, Paz S, Fainboim H, Podestá LG, Fainboim L
Frontiers in immunology 2017;8:1912

Changes in peripheral immune cell numbers and functions in octogenarian walkers - an acute exercise study.

van der Geest KSM, Wang Q, Eijsvogels TMH, Koenen HJP, Joosten I, Brouwer E, Hopman MTE, Jacobs JFM, Boots AMH
Immunity & ageing : I & A 2017;14:5

Enhancement of cytokine-driven NK cell IFN-γ production after vaccination of HCMV infected Africans.

Darboe A, Danso E, Clarke E, Umesi A, Touray E, Wegmuller R, Moore SE, Riley EM, Goodier MR
European journal of immunology 2017 Jun;47(6):1040-1050

Antibody-Dependent NK Cell Activation Differentially Targets EBV-Infected Cells in Lytic Cycle and Bystander B Lymphocytes Bound to Viral Antigen-Containing Particles.

López-Montañés M, Alari-Pahissa E, Sintes J, Martínez-Rodríguez JE, Muntasell A, López-Botet M
Journal of immunology (Baltimore, Md. : 1950) 2017 Jul 15;199(2):656-665

Elevated Neopterin Levels Predict Early Death in Older Hip-fracture Patients.

Larsen M, Bayard C, Lepetitcorps H, Cohen-Bittan J, Appay V, Boddaert J, Sauce D
EBioMedicine 2017 Dec;26:157-164

New insights on the natural killer cell repertoire from a thorough analysis of cord blood cells.

Rettman P, Willem C, David G, Riou R, Legrand N, Esbelin J, Cesbron A, Senitzer D, Gagne K, Retière C
Journal of leukocyte biology 2016 Sep;100(3):471-9

CD2-CD58 interactions are pivotal for the activation and function of adaptive natural killer cells in human cytomegalovirus infection.

Rölle A, Halenius A, Ewen EM, Cerwenka A, Hengel H, Momburg F
European journal of immunology 2016 Oct;46(10):2420-2425

Influenza Vaccination Generates Cytokine-Induced Memory-like NK Cells: Impact of Human Cytomegalovirus Infection.

Goodier MR, Rodriguez-Galan A, Lusa C, Nielsen CM, Darboe A, Moldoveanu AL, White MJ, Behrens R, Riley EM
Journal of immunology (Baltimore, Md. : 1950) 2016 Jul 1;197(1):313-25

Natural Killer p46 Controls Hepatitis B Virus Replication and Modulates Liver Inflammation.

Li W, Jiang Y, Wang X, Jin J, Qi Y, Chi X, Zhang H, Feng X, Niu J
PloS one 2015;10(8):e0135874

Up-regulation of activating and inhibitory NKG2 receptors in allogeneic and autologous hematopoietic stem cell grafts.

Picardi A, Mengarelli A, Marino M, Gallo E, Benevolo M, Pescarmona E, Cocco R, Fraioli R, Tremante E, Petti MC, De Fabritiis P, Giacomini P
Journal of experimental & clinical cancer research : CR 2015 Sep 11;34:98

The Human NK Cell Response to Yellow Fever Virus 17D Is Primarily Governed by NK Cell Differentiation Independently of NK Cell Education.

Marquardt N, Ivarsson MA, Blom K, Gonzalez VD, Braun M, Falconer K, Gustafsson R, Fogdell-Hahn A, Sandberg JK, Michaëlsson J
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 1;195(7):3262-72

Impaired NK Cell Responses to Pertussis and H1N1 Influenza Vaccine Antigens in Human Cytomegalovirus-Infected Individuals.

Nielsen CM, White MJ, Bottomley C, Lusa C, Rodríguez-Galán A, Turner SE, Goodier MR, Riley EM
Journal of immunology (Baltimore, Md. : 1950) 2015 May 15;194(10):4657-67

Human CD8+ T-cells recognizing peptides from Mycobacterium tuberculosis (Mtb) presented by HLA-E have an unorthodox Th2-like, multifunctional, Mtb inhibitory phenotype and represent a novel human T-cell subset.

van Meijgaarden KE, Haks MC, Caccamo N, Dieli F, Ottenhoff TH, Joosten SA
PLoS pathogens 2015 Mar;11(3):e1004671

Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells.

Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson MA, Kekäläinen E, Johansson H, Mjösberg J, Westgren M, Lankisch TO, Wedemeyer H, Ellis EC, Ljunggren HG, Michaëlsson J, Björkström NK
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 15;194(6):2467-71

2DL1, 2DL2 and 2DL3 all contribute to KIR phenotype variability on human NK cells.

Dunphy SE, Guinan KJ, Chorcora CN, Jayaraman J, Traherne JA, Trowsdale J, Pende D, Middleton D, Gardiner CM
Genes and immunity 2015 Jul-Aug;16(5):301-10

NK Cell and Ig Interplay in Defense against Herpes Simplex Virus Type 1: Epistatic Interaction of CD16A and IgG1 Allotypes of Variable Affinities Modulates Antibody-Dependent Cellular Cytotoxicity and Susceptibility to Clinical Reactivation.

Moraru M, Black LE, Muntasell A, Portero F, López-Botet M, Reyburn HT, Pandey JP, Vilches C
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 15;195(4):1676-84

Elevated plasma soluble CD14 and skewed CD16+ monocyte distribution persist despite normalisation of soluble CD163 and CXCL10 by effective HIV therapy: a changing paradigm for routine HIV laboratory monitoring?

Castley A, Berry C, French M, Fernandez S, Krueger R, Nolan D
PloS one 2014;9(12):e115226

Tracking in vivo dynamics of NK cells transferred in patients undergoing stem cell transplantation.

Killig M, Friedrichs B, Meisig J, Gentilini C, Blüthgen N, Loddenkemper C, Labopin M, Basara N, Pfrepper C, Niederwieser DW, Uharek L, Romagnani C
European journal of immunology 2014 Sep;44(9):2822-34

Dopamine inhibits the effector functions of activated NK cells via the upregulation of the D5 receptor.

Mikulak J, Bozzo L, Roberto A, Pontarini E, Tentorio P, Hudspeth K, Lugli E, Mavilio D
Journal of immunology (Baltimore, Md. : 1950) 2014 Sep 15;193(6):2792-800

Long-term effect on natural killer cells by interferon-α therapy on the outcomes of HCV infection.

Hu X, Jiang Y, Li X, Gao Y, Guo X, Chi X, Yan H, Feng J, Zhong J, Sun B, Shao X, Xiao W, Pan Y, Niu J
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2014 May;34(5):366-75

Expansion of CMV-mediated NKG2C+ NK cells associates with the development of specific de novo malignancies in liver-transplanted patients.

Achour A, Baychelier F, Besson C, Arnoux A, Marty M, Hannoun L, Samuel D, Debré P, Vieillard V, K-GREF Study Group.
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 1;192(1):503-11

Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection.

Charoudeh HN, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M
European journal of immunology 2013 Feb;43(2):480-7

Natural killer cells are characterized by the concomitantly increased interferon-γ and cytotoxicity in acute resolved hepatitis B patients.

Zhao J, Li Y, Jin L, Zhang S, Fan R, Sun Y, Zhou C, Shang Q, Li W, Zhang Z, Wang FS
PloS one 2012;7(11):e49135

Human cytomegalovirus (CMV)-induced memory-like NKG2C(+) NK cells are transplantable and expand in vivo in response to recipient CMV antigen.

Foley B, Cooley S, Verneris MR, Curtsinger J, Luo X, Waller EK, Anasetti C, Weisdorf D, Miller JS
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 15;189(10):5082-8

CMV drives clonal expansion of NKG2C+ NK cells expressing self-specific KIRs in chronic hepatitis patients.

Béziat V, Dalgard O, Asselah T, Halfon P, Bedossa P, Boudifa A, Hervier B, Theodorou I, Martinot M, Debré P, Björkström NK, Malmberg KJ, Marcellin P, Vieillard V
European journal of immunology 2012 Feb;42(2):447-57

NKG2A inhibits NKG2C effector functions of γδ T cells: implications in health and disease.

Angelini DF, Zambello R, Galandrini R, Diamantini A, Placido R, Micucci F, Poccia F, Semenzato G, Borsellino G, Santoni A, Battistini L
Journal of leukocyte biology 2011 Jan;89(1):75-84

Analysis of CD16+CD56dim NK cells from CLL patients: evidence supporting a therapeutic strategy with optimized anti-CD20 monoclonal antibodies.

Le Garff-Tavernier M, Decocq J, de Romeuf C, Parizot C, Dutertre CA, Chapiro E, Davi F, Debré P, Prost JF, Teillaud JL, Merle-Beral H, Vieillard V
Leukemia 2011 Jan;25(1):101-9

Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.

Björkström NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaëlsson J, Malmberg KJ, Klingström J, Ahlm C, Ljunggren HG
The Journal of experimental medicine 2011 Jan 17;208(1):13-21

Shaping of iNKT cell repertoire after unrelated cord blood transplantation.

Beziat V, Nguyen S, Exley M, Achour A, Simon T, Chevallier P, Sirvent A, Vigouroux S, Debré P, Rio B, Vieillard V, French Minicord Study Group.
Clinical immunology (Orlando, Fla.) 2010 Jun;135(3):364-73

High log-scale expansion of functional human natural killer cells from umbilical cord blood CD34-positive cells for adoptive cancer immunotherapy.

Spanholtz J, Tordoir M, Eissens D, Preijers F, van der Meer A, Joosten I, Schaap N, de Witte TM, Dolstra H
PloS one 2010 Feb 15;5(2):e9221

NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs.

Béziat V, Descours B, Parizot C, Debré P, Vieillard V
PloS one 2010 Aug 6;5(8):e11966

The decreased expression of Siglec-7 represents an early marker of dysfunctional natural killer-cell subsets associated with high levels of HIV-1 viremia.

Brunetta E, Fogli M, Varchetta S, Bozzo L, Hudspeth KL, Marcenaro E, Moretta A, Mavilio D
Blood 2009 Oct 29;114(18):3822-30

An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.

Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socié G, Toubert A
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 1;181(3):2227-37

Establishment and characterization of SRIK-NKL: a novel CD8+ natural killer/T cell line derived from a patient with leukemic phase of acute lymphoblastic lymphoma.

Srivastava BI, Srivastava MD
Leukemia research 2005 Jul;29(7):771-83

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Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of NKG2C/CD159c in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2C/CD159c PE-conjugated Monoclonal Antibody (Catalog # FAB138P) or (B) Mouse IgG₁ Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.