Explore
Validate
Learn
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF546 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal TSLPR/CRLF2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse TSLP R in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Immune Characterization of Bone Marrow-Derived Models of Mucosal and Connective Tissue Mast Cells.
TSLP Protects Corneas From Pseudomonas aeruginosa Infection by Regulating Dendritic Cells and IL-23-IL-17 Pathway.
IL-7Rα Expression Regulates Murine Dendritic Cell Sensitivity to Thymic Stromal Lymphopoietin.
Rearrangement of CRLF2 in B-progenitor- and Down syndrome-associated acute lymphoblastic leukemia.
Overexpression of murine TSLP impairs lymphopoiesis and myelopoiesis.
Benedé S, Cody E, Agashe C, Berin MC
Allergy, asthma & immunology research 2018 May;10(3):268-277
Allergy, asthma & immunology research 2018 May;10(3):268-277
TSLP Protects Corneas From Pseudomonas aeruginosa Infection by Regulating Dendritic Cells and IL-23-IL-17 Pathway.
Cui X, Gao N, Me R, Xu J, Yu FX
Investigative ophthalmology & visual science 2018 Aug 1;59(10):4228-4237
Investigative ophthalmology & visual science 2018 Aug 1;59(10):4228-4237
IL-7Rα Expression Regulates Murine Dendritic Cell Sensitivity to Thymic Stromal Lymphopoietin.
Kummola L, Ortutay Z, Chen X, Caucheteux S, Hämäläinen S, Aittomäki S, Yagi R, Zhu J, Pesu M, Paul WE, Junttila IS
Journal of immunology (Baltimore, Md. : 1950) 2017 May 15;198(10):3909-3918
Journal of immunology (Baltimore, Md. : 1950) 2017 May 15;198(10):3909-3918
Rearrangement of CRLF2 in B-progenitor- and Down syndrome-associated acute lymphoblastic leukemia.
Mullighan CG, Collins-Underwood JR, Phillips LA, Loudin MG, Liu W, Zhang J, Ma J, Coustan-Smith E, Harvey RC, Willman CL, Mikhail FM, Meyer J, Carroll AJ, Williams RT, Cheng J, Heerema NA, Basso G, Pession A, Pui CH, Raimondi SC, Hunger SP, Downing JR, Carroll WL, Rabin KR
Nature genetics 2009 Nov;41(11):1243-6
Nature genetics 2009 Nov;41(11):1243-6
Overexpression of murine TSLP impairs lymphopoiesis and myelopoiesis.
Osborn MJ, Ryan PL, Kirchhof N, Panoskaltsis-Mortari A, Mortari F, Tudor KS
Blood 2004 Feb 1;103(3):843-51
Blood 2004 Feb 1;103(3):843-51
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TSLP R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF546, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP R Antibody. Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with mouse IL-7 R alpha/CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF546). The ND50 is typically 0.2-0.6 µg/mL.
