PA5-30160

antibody from Invitrogen Antibodies

Targeting: RPS2 LLREP3, S2

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-30160 - Provider product page
Provider: Invitrogen Antibodies
Product name: RPS2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, Jurkat, Raji, mouse brain. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (99%), Drosophila (85%), Xenopus laevis (98%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Zebrafish
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.2 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RPS2 protein structure - PA5-30160 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2) and NIH/3T3 (Lane 3). The blot was probed with Anti-RPS2 Polyclonal Antibody (Product # PA5-30160, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 34 kDa band corresponding to RPS2 was observed across the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using RPS2 Polyclonal Antibody (Product # PA5-30160). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. 12% SDS PAGE. RPS2 Polyclonal Antibody (Product # PA5-30160) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using RPS2 Polyclonal Antibody (Product # PA5-30160). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. RPS2 Polyclonal Antibody (Product # PA5-30160) diluted at 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of RPS2 was achieved by transfecting HeLa cells with RPS2 specific siRNAs (Silencer® select Product # s12254, s12253). Western blot analysis (Fig. a) was performed using whole cell extracts from the RPS2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with RPS2 Polyclonal Antibody (Product # PA5-30160, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RPS2.