Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 17776-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#17776-1-AP, RRID:AB_2217085
- Product name
- VPS16 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated VPS16 antibody (Cat. #17776-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.
Regulation of TRPML1 channel activity and inflammatory exosome release by endogenously produced reactive oxygen species in mouse podocytes.
Contribution of podocyte inflammatory exosome release to glomerular inflammation and sclerosis during hyperhomocysteinemia.
Abnormal podocyte TRPML1 channel activity and exosome release in mice with podocyte-specific Asah1 gene deletion.
Label-Free Quantitative Proteomic Profiling of LAD2 Mast Cell Releasates Reveals the Mechanism of Tween-80-Induced Anaphylactoid Reaction.
Control of lysosomal TRPML1 channel activity and exosome release by acid ceramidase in mouse podocytes.
Lysosomal regulation of extracellular vesicle excretion during d-ribose-induced NLRP3 inflammasome activation in podocytes.
Autophagosome maturation mediated by Rab7 contributes to neuroprotection of hypoxic preconditioning against global cerebral ischemia in rats.
Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.
Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.
Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.
Distinct autophagosomal-lysosomal fusion mechanism revealed by thapsigargin-induced autophagy arrest.
Shen Q, Shi Y, Liu J, Su H, Huang J, Zhang Y, Peng C, Zhou T, Sun Q, Wan W, Liu W
Autophagy 2021 May;17(5):1157-1169
Autophagy 2021 May;17(5):1157-1169
Regulation of TRPML1 channel activity and inflammatory exosome release by endogenously produced reactive oxygen species in mouse podocytes.
Li G, Huang D, Li N, Ritter JK, Li PL
Redox biology 2021 Jul;43:102013
Redox biology 2021 Jul;43:102013
Contribution of podocyte inflammatory exosome release to glomerular inflammation and sclerosis during hyperhomocysteinemia.
Huang D, Li G, Zhang Q, Bhat OM, Zou Y, Ritter JK, Li PL
Biochimica et biophysica acta. Molecular basis of disease 2021 Jul 1;1867(7):166146
Biochimica et biophysica acta. Molecular basis of disease 2021 Jul 1;1867(7):166146
Abnormal podocyte TRPML1 channel activity and exosome release in mice with podocyte-specific Asah1 gene deletion.
Li G, Huang D, Bhat OM, Poklis JL, Zhang A, Zou Y, Kidd J, Gehr TWB, Li PL
Biochimica et biophysica acta. Molecular and cell biology of lipids 2021 Feb;1866(2):158856
Biochimica et biophysica acta. Molecular and cell biology of lipids 2021 Feb;1866(2):158856
Label-Free Quantitative Proteomic Profiling of LAD2 Mast Cell Releasates Reveals the Mechanism of Tween-80-Induced Anaphylactoid Reaction.
Mi YN, Yan PP, Di J, Cao L, Xiao X, Liu DZ, Cao YX
Proteomics. Clinical applications 2020 Jul;14(4):e1900065
Proteomics. Clinical applications 2020 Jul;14(4):e1900065
Control of lysosomal TRPML1 channel activity and exosome release by acid ceramidase in mouse podocytes.
Li G, Huang D, Hong J, Bhat OM, Yuan X, Li PL
American journal of physiology. Cell physiology 2019 Sep 1;317(3):C481-C491
American journal of physiology. Cell physiology 2019 Sep 1;317(3):C481-C491
Lysosomal regulation of extracellular vesicle excretion during d-ribose-induced NLRP3 inflammasome activation in podocytes.
Hong J, Bhat OM, Li G, Dempsey SK, Zhang Q, Ritter JK, Li W, Li PL
Biochimica et biophysica acta. Molecular cell research 2019 May;1866(5):849-860
Biochimica et biophysica acta. Molecular cell research 2019 May;1866(5):849-860
Autophagosome maturation mediated by Rab7 contributes to neuroprotection of hypoxic preconditioning against global cerebral ischemia in rats.
Zhan L, Chen S, Li K, Liang D, Zhu X, Liu L, Lu Z, Sun W, Xu E
Cell death & disease 2017 Jul 20;8(7):e2949
Cell death & disease 2017 Jul 20;8(7):e2949
Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.
van der Kant R, Jonker CT, Wijdeven RH, Bakker J, Janssen L, Klumperman J, Neefjes J
The Journal of biological chemistry 2015 Dec 18;290(51):30280-90
The Journal of biological chemistry 2015 Dec 18;290(51):30280-90
Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.
Ulbricht A, Eppler FJ, Tapia VE, van der Ven PF, Hampe N, Hersch N, Vakeel P, Stadel D, Haas A, Saftig P, Behrends C, Fürst DO, Volkmer R, Hoffmann B, Kolanus W, Höhfeld J
Current biology : CB 2013 Mar 4;23(5):430-5
Current biology : CB 2013 Mar 4;23(5):430-5
Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.
van der Kant R, Fish A, Janssen L, Janssen H, Krom S, Ho N, Brummelkamp T, Carette J, Rocha N, Neefjes J
Journal of cell science 2013 Aug 1;126(Pt 15):3462-74
Journal of cell science 2013 Aug 1;126(Pt 15):3462-74
Distinct autophagosomal-lysosomal fusion mechanism revealed by thapsigargin-induced autophagy arrest.
Ganley IG, Wong PM, Gammoh N, Jiang X
Molecular cell 2011 Jun 24;42(6):731-43
Molecular cell 2011 Jun 24;42(6):731-43
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 17776-1-AP(VPS16 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human liver using 17776-1-AP(VPS16 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The VPS16 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human VPS16. This antibody recognizes human,mouse,rat antigen. The VPS16 antibody has been validated for the following applications: ELISA, WB, IHC analysis.