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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-58113 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNX18 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DDEWDDSSTV ADEPGALGSG AYPDLDGSSS AGVGAAGRYR LSTRSDLSLG SRGGSVPPQH HPSGPKSSAT VSRNLN
- Concentration
- 0.1 mg/mL
Submitted references N-WASP Control of LPAR1 Trafficking Establishes Response to Self-Generated LPA Gradients to Promote Pancreatic Cancer Cell Metastasis.
Juin A, Spence HJ, Martin KJ, McGhee E, Neilson M, Cutiongco MFA, Gadegaard N, Mackay G, Fort L, Lilla S, Kalna G, Thomason P, Koh YWH, Norman JC, Insall RH, Machesky LM
Developmental cell 2019 Nov 18;51(4):431-445.e7
Developmental cell 2019 Nov 18;51(4):431-445.e7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNX18 in human cell line BEWO using a SNX18 Polyclonal Antibody (Product # PA5-58113).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNX18 in mouse cell line NIH-3T3 and rat cell line NBT-II using a SNX18 Polyclonal Antibody (Product # PA5-58113).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SNX18 in human cell line U-251 MG shows positivity in vesicles & nucleus but excluded from the nucleoli. Samples were probed using a SNX18 Polyclonal Antibody (Product # PA5-58113).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SNX18 in human cell line A-431 using a SNX18 Polyclonal Antibody (Product # PA5-58113) shows localization to cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SNX18 in human placenta shows strong cytoplasmic positivity in trophoblastic cells. Samples were probed using a SNX18 Polyclonal Antibody (Product # PA5-58113).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 The N-WASP-SNX18 Complex Regulates LPAR1 Recycling and Chemotaxis in PDAC Cells (A) Volcano plot showing proteins binding to GFP-N-WASP in 15N (rescued cells) versus GFP in 15G (N-WASP knockout cells) after 15 min LPA stimulation (t test, p
