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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF7548 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Caspr1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Caspr1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human Caspr2 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P78357- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Phenotypic heterogeneity and plasticity of isocortical and hippocampal astrocytes in the human brain.
Sosunov AA, Wu X, Tsankova NM, Guilfoyle E, McKhann GM 2nd, Goldman JE
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Feb 5;34(6):2285-98
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Feb 5;34(6):2285-98
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Caspr1 in Human Brain. Caspr1 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human Caspr1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7548) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to paranodal axonal regions in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
