HPA050574

antibody from Atlas Antibodies

Targeting: MAK16 MAK16L, RBM13

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA050574

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA050574, RRID:AB_2681181

Product name

Anti-MAK16

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

FIRHKCKQRFTKITQYLIRIRKLTLKRQRKLVPLS
KKVERREKRREEKALIAAQLDNAIEKELLERLKQD
TYGDIYNFPIHAFDKALEQQ


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and MAK16 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410084).

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & vesicles.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum shows distinct nucleolar positivity in Purkinje cells.

Sample type

HUMAN