Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- BS-3615R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ESM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90 min in 37°C. Predicted band 20 kD. Observed band size: 20 kD.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed and human kidney tissue labeled with Anti-ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.