AP14219a

antibody from Abgent

Targeting: HMGN1 FLJ27265, FLJ31471, HMG14, MGC104230, MGC117425

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP14219a

Provider

Abgent

Proper citation

Abgent Cat#AP14219a, RRID:AB_11134394

Product name

HMGN1 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

HMGN1 Antibody (N-term) (Cat. #AP14219a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HMGN1 antibody detected the HMGN1 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of HMGN1 (arrow) using rabbit polyclonal HMGN1 Antibody (N-term) (Cat. #AP14219a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMGN1 gene.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

HMGN1 Antibody (N-term) (AP14219a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HMGN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50