Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP07722PU-N - Provider product page
- Provider
- OriGene
- Product name
- HMG14 (HMGN1) pSer20/24 (19-28) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- HMG14 (HMGN1) pSer20/24 (19-28) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- HMGN1
- Antibody clone number
- NULL
- Vial size
- 50 µg
- Concentration
- 1 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-HMNG Antibody - Western Blot. Western blot of Affinity Purified anti-HMNG antibody shows detection of phosphorylated HMGN1 and HMGN2. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues. Lanes contain: 1 - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1000 for this experiment. The blot was procebetaed using a 5 sec exposure time. Personal Communication, Yuri Postnikov, NIH, CCR, Bethesda, MD.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-Fixed Paraffin-Embedded Human Skin stained with Anti-HMGN1 antibody at 20 ug/ml after heat-induced antigen retrieval.
- Validation comment
- IHC
