NBP1-78102

antibody from Novus Biologicals

Targeting: HMGN1 FLJ27265, FLJ31471, HMG14, MGC104230, MGC117425

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-78102

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-78102, RRID:AB_11007560

Product name

Rabbit Polyclonal HMGN1/HMG14 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This antibody is directed against phosphorylated human HMGN protein at pS20 and pS24 residues (other HMGN proteins have this conserved sequence but at other residue positions).

Reactivity

Human, Bovine, Chicken/Avian, Sheep

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.2 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Damage-Associated Molecular Pattern and Fetal Membrane Vascular Injury and Collagen Disorganization in Lipopolysaccharide-Induced Intra-amniotic Inflammation in Fetal Sheep.

Regan JK, Kannan PS, Kemp MW, Kramer BW, Newnham JP, Jobe AH, Kallapur SG
Reproductive sciences (Thousand Oaks, Calif.) 2016 Jan;23(1):69-80

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1,000 for this experiment. The blot was processed using a 5 sec exposure time.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - Used at 20 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.