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LearnAP09301PU-N
antibody from Acris Antibodies GmbH
Targeting: NOXO1
P41NOXA, P41NOXB, P41NOXC, SH3PXD5, SNX28
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP09301PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09301PU-N, RRID:AB_2035802
- Product name
- anti NOXO1 (238-252)
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein corresponding to amino acids 238-252 of Human NOXO1 protein
- Reactivity
- Human, Canine, Guinea Pig
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
Submitted references HSP90 inhibition by 17-DMAG attenuates oxidative stress in experimental atherosclerosis.
Madrigal-Matute J, Fernandez-Garcia CE, Gomez-Guerrero C, Lopez-Franco O, Muñoz-Garcia B, Egido J, Blanco-Colio LM, Martin-Ventura JL
Cardiovascular research 2012 Jul 1;95(1):116-23
Cardiovascular research 2012 Jul 1;95(1):116-23
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using anti-NOXO1 antibody shows detection of a band ~50 kDa corresponding to Human NOXO1 (arrowhead). Reactivity was observed in transfected Human 293 cells and Human HT-29 colon carcinoma cells (endogenous). Under these conditions endogenous NOXO1 detection was not observed in HeLa, HL-60, untransfected 293 or WT MEF cells. A 1/1,000 dilution of the primary antibody was used for detection followed by secondary antibody reactivity. Specific band reactivity was competed away when the antibody was pre-incubated with the peptide immunogen (data not shown). Personal Communication, Zhenggang Liu, NIH, CCR, Bethesda, MD.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry. Anti-NOXO1 antibody was used at 5 µg/ml to detect signal in a variety of tissues including multi-Human, multi-brain and multi-cancer slides. This image shows moderate positive staining of the lamina propia in Human colon epithelium and macrophages at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle,WA.
