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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunoprecipitation [1]
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- Product number
- NB100-1575 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1575, RRID:AB_2187475
- Product name
- Rabbit Polyclonal SKIV2L2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Tri-snRNP-associated proteins interact with subunits of the TRAMP and nuclear exosome complexes, linking RNA decay and pre-mRNA splicing.
Nag A, Steitz JA
RNA biology 2012 Mar;9(3):334-42
RNA biology 2012 Mar;9(3):334-42
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: SKIV2L2 Antibody [NB100-1575] - Detection of Human SKIV2L2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SKIV2L2 antibody NB100-1575 used for IP at 3 mcg/mg lysate. SKIV2L2 was unsuccessfully immunoprecipitated using anti-SKIV2L2 antibody NB100-1574 at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.
