Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ANT-189-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC38A9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membrane (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1-2. Anti-SLC38A9 Antibody (#ANT-189), (1:200). 3-4. Anti-SLC38A9 Antibody , preincubated with SLC38A9 Blocking Peptide (#BLP-NT189).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human HepG2 hepatocellular carcinoma cell line lysate (lanes 1 and 4), human MCF-7 breast adenocarcinoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6): - 1-3. Anti-SLC38A9 Antibody (#ANT-189), (1:200). 4-6. Anti-SLC38A9 Antibody , preincubated with SLC38A9 Blocking Peptide (#BLP-NT189).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of SLC38A9 in mouse cingulate cortex. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections Anti-SLC38A9 Antibody (#ANT-189), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. SLC38A9 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the Antibody with SLC38A9 Blocking Peptide (#BLP-NT189), suppressed staining. Cell nuclei are stained with DAPI (blue).