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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10623 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10623, RRID:AB_11188572
- Product name
- DDX4 monoclonal antibody, clone 2F9H5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant DDX4.
- Isotype
- IgG
- Antibody clone number
- 2F9H5
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) against DDX4-hIgGFc transfected HEK293 cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MSCs (upper) and NTERA-2 (bottom) cells using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and rectal cancer (bottom) , showing cytoplasmic localization using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MSCs cells using DDX4 monoclonal antibody, clone 2F9H5 (Cat # MAB10623) (green) and negative control (purple) .
- Validation comment
- Flow Cytometry
