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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 13873-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DGKH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAVSRVIKL QHHRIAQCRT VKITIFGDEG VPVQVDGEAW VQPPGIIKIV HKNRAQMLTR DRAFESTLKS WEDKQKCDSG KPVLRTHLYI HHAIDLATEE VSQMQLCSQA AEELITRICD AATIHCLLEQ ELAHAVNACS HALNKANPRC PESLTRDTAT EIAINVKALY NETESLLVGR VPLQLESPHE ERVSNALHSV EVELQKLTEI PWLYYILHPN EDEEPPMDCT KRNNRSTVFR IVPKFKKEKV QKQKTSSQPV QKWGTEEVAA WLDLLNLGEY KDIFIRHDIR GAELLHLERR DLKNTVGEKR DTKENGKHMD LGIPKVGHVK RILQGIKELG RSTPQSEV (754-1100 aa encoded by BC043292)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13873-1-AP (DGKH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13873-1-AP (DGKH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Acetone ) fixed SH-SY5Y cells using 13873-1-AP (DGKH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 13873-1-AP ( DGKH Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 13873-1-AP ( DGKH Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DGKH (IP:13873-1-AP, 5ug; Detection:13873-1-AP 1:500) with mouse brain tissue lysate 2640ug.
